Description
Test Principle: This assay employs a two-site sandwich ELISA to quantitate FBXL16 in samples. An antibody specific for FBXL16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FBXL16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FBXL16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBXL16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Members of the F-box protein family, such as FBXL16, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains. Jin et al. (2004) reported that the human FBXL16 protein contains an F box in its N-terminal half, followed by a spacer, 8 tandemly arrayed leucine-rich repeats, another spacer, and a C-terminal leucine-rich repeat.By sequence analysis, Daniels et al. (2001) mapped the FBXL16 gene, which they called C16ORF22, to a gene-rich region of chromosome 16p13.3.Jin et al. (2004) stated that the mouse Fbxl16 gene maps to chromosome 17A3.3